Gc-rich pcr system roche manual

5 degrees less than the manual annealing temp with DMSO n 20 degrees less if u using formamide____20sec. gc rich pcr system from Roche, as is. Cite. 1 . GC-RICH PCR System sufficient for ≤50 reactions, pkg of U, optimum reaction temp. 72 °C; Synonyms: GC-rich | PCR; find Roche MSDS, related peer-reviewed papers, technical documents, similar products more at Sigma-Aldrich. concentrations include long PCR (10 kb) and AT-rich PCR, as well as amplification using primers with a low GC content (GC-rich PCR Use KAPA HiFi GC Buffer for the amplification of GC-rich targets. Alternatively, evaluate the KAPA HiFi Fidelity Buffer + 5% DMSO. Should neither of these result in.

PCR product Difficult template e.g., GC-rich templates † Perform initial denaturation step at 95°C for 3 - 5 min. † Use the GC-RICH PCR System*. Poor DNA template quality Check quality and concentration of template: † Analyze an aliquot on an agarose gel to check for possible degrada-tion. † Include a control reaction using a. UNSPSC Code: Components: GC-RICH Enzyme Mix, in storage buffer; GC-RICH PCR Reaction Buffer, with mM MgCl2 and DMSO 5x concentrated; GC-RICH Resolution Solution 5 M; MgCl2 Stock Solution. The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity.

Taq DNA Polymerase available from Roche Applied Science) are inactive at low tempera- of GC-rich sequences with high yield, the GC-RICH PCR System* from. fidelity of KAPA2G Fast is similar to that of wild-type Taq; Amplification of highly GC-rich fragments (>70%) Standard PCR Protocol. IMPORTANT! 14 ພ.ພ. GeneAmp® is a registered trademark of Roche Molecular Systems, Inc., For amplifying GC-rich templates: The Advantage-GC 2 PCR Kit.